Aptamer‐Modified Nanohydrogel Microarrays for Bioselective Cancer Cell Immobilization

Photolithography combined with surface nucleated protein self-assembly of azido-modified spider silk proteins is used to create an arbitrarily shaped, inherently cell repellent micropattern based on nanofibrillar networks. Using “click” chemistry dibenzocyclooctin modified oligonucleotides, the microstructures are functionalized DNA-aptamers, which selectively bind cancer markers tyrosine kinase 7 or nucleolin. The epitope-specific interaction aptamer-modified surfaces tested using human non-adherend leukemia T cells (Jurkat), as well adherent cervix carcinoma (HeLa) and neuroblastoma (Kelly) cells. can be immobilized high precision densities pattern, also revealing spatially defined proliferation spreading into distinct morphologies upon cultivation. formation integrin-based focal adhesions occurs in case aptamer cells, similarly those anchored RGD-modified pattern. firm aptamer-marker anchorage allows for integrin-dependent adhesions. Due amenability recombinant towards chemical genetical modifications, presented micropatterned fibrous networks have great potential further development adjustable biocompatible cell-specific arrays, enabling applications circulating isolation cultivation, studies cell's pathogenesis, progression metastasis capabilities platforms personalized medicine.